LemBix Lab Techniques

Molecular Biology: cDNA library construction, Real-time PCR, microarrays, subtractive hybridization, knock-in mouse and transgenic mouse generation, mouse genetics, gene mapping

Microscopy: time-lapse, FRET, TIRF, High Content Screening

Cell Culture: primary culture of neurons (cortical, hippocampal, cerebellar granule neurons, DRGs, spinal cord), hybridoma production, transfection of primary neurons, culture of primary astrocytes and Schwann cells

Biochemistry: HPLC, affinity purifications