The Laboratory for Axon Growth and GuidenceNeuron Red Growth Cones
 

 

We have only used the antibodies from Covance, BD Pharmingen and The DSHB and can not comment on the usefulness of the antibodies from other sources.

BD Pharmingen™
CD171 (L1 Neural Cell Adhesion Molecule) 5G3, From Ralph Reisfeld at Scripps, to human L1. Good for IHC and IPs, not so good for westerns.

Covance Research Products
L1 NCAM Monoclonal Antibody, MMS-172R This antibody binds to the cytoplasmic domain of L1 in mammals and birds. It is from our lab. It does not bind if the L1 is phosphorylated at Y1176. It works on western blots and for IHC on Bouin's fixed tissue and cells. Good for IPs

Developmental Studies Hybridoma Bank
8D9 - mouse monoclonal to chicken NgCAM, good for IHC and IPs, not for westerns. It is from our lab.
ASCS4 - mouse monoclonal to Rat L1, good for IPs, no experience with IHC or westerns. It is from Paul Patterson.

No experience with the following antibodies

BD Biosciences Pharmingen
Mouse Anti-CD171 (L1 Adhesion Molecule) Monoclonal Antibody , Clone 17 Reactivity: Mouse, Rat, Human, Canine, Bovine, Chicken

Novus Biologicals
Mouse Anti-L1CAM Monoclonal Antibody, Unconjugated , Clone UJ127, Reactivity: Human

CHEMICON
Rat Anti-L1 Monoclonal Antibody, Unconjugated , Clone 324, Reactivity: Mouse; Rat
Mouse Anti-L1 NCAM Monoclonal Antibody, Unconjugated , Clone UJ127.11, Reactivity: Human

QED Bioscience Inc.
Mouse Anti-Human L1-CAM Monoclonal Antibody, Unconjugated , Clone N-A8, Reactivity: Human, porcine and chicken

United States Biological
Rat Anti-Mouse L1 Monoclonal Antibody, Unconjugated , Clone 2Q8, Reactivity: Mouse
Mouse Anti-Human L1 Cell Adhesion Molecule (L1CAM) Monoclonal Antibody , Clone 0.N.378, Reactivity: Human

Lab Vision
Mouse Anti-Human L1 Cell Adhesion Molecule Ab-1 Monoclonal Antibody, Biotin Labeled, Clone UJ127, Reactivity: Human. Others-not known.

Serotec
CD171, mouse IgG1 clone UJ127.11, F,C,IP, WB

Santa Cruz Biotech
NCAM-L1 (C-20), sc-1508, goat IgG, C-terminus (h), WB, IP, IHC, m, r, h
NCAM-L1 (H-200), sc-15326, rabbit IgG, 921-1120 (h), WB, IP, IHC, m, r, h

Acris Antibodies
anti human CD171, UJ127.11, mouse IgG1

Research Diagnostics Inc
CD171

 

 
University of Miami Miller School of Medicine
 
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Dr. Vance Lemmon and Dr. John Bixby
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